Rahmat Kurniawan, Melysa Saleh, Arif Ashari, Hawa Purnama, Muhammad Yogi Saputra, Tati Suhartati, Sukrasno Sukrasno
Callistemon citrinus is an aromatic herb, its lemon-scented leaves and bioactive potential propose that it could find a niche as a flavoring spice, intensely in herbal teas and natural medicine formulations. The C. citrinus from ITERA Botanical Gardens is reported to contain α-amyrin, a triterpene with promising bioactivity, prevalent in various segments of C. citrinus. The α-amyrin has cytotoxic properties, which are of interest in both medicinal and food science topics. This study objects to isolate and measure the α-amyrin content from various parts of C. citrinus. The α-amyrin exhibited high concentrations in n-hexane bark extract by 39.27 ± 0.774 ppm and showed moderate-weak activity against A549, MCF7, and HeLa, with IC50 values of 54.14 ± 1.41, 82.79 ± 0.86, 69.35 ± 2.37 µM respectively. This research intends to support the Botanical Garden’s greening campaign by investigating bioactive compounds against malignant human cancer cells. © 2025, Walailak University. All rights reserved.
Departement Chemistry Science, Institut Teknologi Sumatera, South Lampung, Indonesia; Departement Chemistry Science, Universitas Lampung, Lampung, Indonesia; Departement Pharmacy Science, Institut Teknologi Sumatera, South Lampung, Indonesia