Corn cob-derived graphene oxide for ciprofloxacin residue determination: Dispersive solid-phase extraction optimization via response surface methodology

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Rinawati, Dian Muthia, Hinaya Viebrienty, Sonny Widiarto, Agung Kiswandono, Fahamsyah H. Latief, Achmad Gus Fahmi, Iwan Syahjoko Saputra

2026 Vacuum Vol. 246 Article Cited by 0 Quartile

Abstract

This study reports the successful synthesis of graphene oxide (GO) from renewable corn cob agricultural waste using a modified Hummers method for its application as an adsorbent in Dispersive Solid Phase Extraction (DSPE) of Ciprofloxacin (CIP), established through the utilization of corn cob-derived GO as a low-cost, sustainable DSPE phase. Characterization confirmed the structural and chemical transformation: Scanning Electron Microscopy (SEM) showed the change from dense graphite particles to highly wrinkled, thin GO nanosheets, indicative of successful exfoliation. X-ray Diffraction (XRD) revealed the complete loss of the sharp graphite (002) peak at 2θ = 26.60° and the emergence of a broad, shifted peak at 2θ approximately 10°, confirming the increased interlayer spacing and the material's amorphous/highly disordered structure due to functional group intercalation. Energy-Dispersive X-ray Spectroscopy (EDX) verified the chemical change, showing a significant increase in oxygen content from 14.76 At% in graphite to 25.08 At% in GO. The DSPE process parameters were optimized using Response Surface Methodology (RSM) with a Box-Behnken Design. The optimal conditions for CIP adsorption were determined to be 20 mg of GO, a pH of 3.75, a CIP concentration of 752.92 ppb, and a contact time of 34.14 min. The GO exhibited a high maximum adsorption percentage of 99.26 %. This work demonstrates the significant potential of corn cob-derived GO as a low-cost, sustainable, and highly effective solid phase for environmental monitoring and extraction of pharmaceutical residues like CIP. © 2025 Elsevier Ltd

Affiliations

Department of Chemistry, Universitas Lampung, Bandar Lampung, 35145, Indonesia; Department of Mechanical Engineering, Universitas Nasional, Jakarta, 12520, Indonesia; Department of Cosmetic Engineering, Faculty of Industrial Technology, Sumatera Institute of Technology, South Lampung, 35365, Indonesia