Panggulu Ahmad Ramadhani Utoro, Dikianur Alvianto, Taufikkilah Romadhon, Agustu Sholeh Pujokaroni, Yulian Andriyani, Maghfirotin Marta Banin, Nur Amaliah, Rimbawan Apriadi, Jatmiko Eko Witoyo, Esti Indri Lestari, Erika Ummiati
Kokang leaves (Lepisanthes amoena), a plant native to Kalimantan, have long been utilized by local communities for treating wounds and maintaining skin health. This species holds promising potential as a natural source of bioactive compounds, particularly due to its antimicrobial and antioxidant constituents that are valuable in both health and food applications. Phenolics and flavonoids are naturally occurring compounds frequently found in plant-derived materials. This study aimed to determine the levels of phenolics, flavonoids, and antioxidant activity in kokang leaf powder extracted using Microwave-Assisted Extraction (MAE). The experimental design incorporated variations in solvent ratios (1:10, 1:15, and 1:20) and extraction durations (2, 3, and 4 minutes). Data were processed using two-way ANOVA at a 95% confidence level. The findings indicated that the interaction between solvent ratio and extraction time significantly influenced the total phenolic content, total flavonoid content, and antioxidant activity of the kokang leaf extract powder. The treatment with a 1:10 solvent ratio and 2-minute extraction time yielded the highest values, including total phenolics (258.6 mg GAE/g), total flavonoids (74.8 mg QE/g), and antioxidant activity (IC50) of 29.4 μg/mL. © 2025 The Authors.
Department of Agricultural Product Technology, Faculty of Agriculture, Mulawarman University, Jl. Tanah Grogot Kampus, Gunung Kelua, 75119, Indonesia; Halal Research Center, Institute for Research and Community Service, Mulawarman University, Jl. Kerayan Kampus, Gunung Kelua, 75119, Indonesia; Department of Agro-Industrial Technology, Faculty of Industrial Technology, Institut Teknologi Sumatera, Jl. Terusan Ryacudu, Desa Way Hui, Kecamatan Jati Agung, Lampung Selatan, 35365, Indonesia