Harlina Ardiyanti, Ni’matil Mabarroh, Nur Aji Wibowo, Nurul Imani Istiqomah, Rivaldo Marsel Tumbelaka, Mahardika Yoga Darmawan, Zurnansyah Zurnansyah, Yuliyan Dwi Prabowo, Moh. Adhib Ulil Absor, Edi Suharyadi
The urgency for cost-effective, rapid, and user-friendly tools for detecting biomolecules is critical due to their essential role in biosensors relying on giant magnetoresistance (GMR) technology. This article presents the performance of a novel configuration for α-amylase assay as the target biomolecule by employing the ICs-based dual chip commercial GMR sensor, which offers a straightforward approach with immediate electrical data output. Magnetite (Fe3O4) magnetic nanoparticles were synthesized using a green approach, employing leaf extract from Moringa oleifera. Meanwhile, the PEG-functionalized green-synthesized Fe3O4 magnetic nanoparticles (Fe3O4/PEG) were used as a magnetic label and produced in an aqua-solution mixture. Each sensor element surface of the dual chip configuration was then exposed to a minute droplet of analyte solution. Each sensor element surface of the dual chip configuration received a little droplet of analyte solution. As a comparison, single chip configuration was also investigated. The microcontroller was connected to the chip, serving as a source-meter unit, facilitating the expeditious production of measurement results. The sensor system demonstrates a consistent and reliable signal that is well-linear to the α-amylase concentration of interest. In a low biasing magnetic field, the signal can be acquired in just 30 s. The dual configuration demonstrated a higher sensitivity in detecting α-amylase (0.98 mV/%) compared to the single chip configuration (0.94 mV/%). A low limit of detection of 3% was also attained. The sensor demonstrated good repeatability and stability, as evidenced by relative signal deviation ranges of 1–20% over 30 s. The dual chip configuration in GMR biosensors is a reliable method for achieving competitive performance with green-synthesized magnetic labels using a low-bias magnetic field. This configuration encourages more responsive detection. The results demonstrate that a facile and fast biomolecule assay method can be achieved by combining the use of a dual chip configuration of GMR sensor and Fe3O4/PEG with a microcontroller. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Department of Physics, Universitas Gadjah Mada, Yogyakarta, Indonesia; Department of Physics, Institut Teknologi Sumatera, Lampung, Indonesia; Department of Physics, Universitas Kristen Satya Wacana, Salatiga, Indonesia
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