Amalia Afifah, Ono Suparno, Liesbetini Haditjaroko, Kustiariyah Tarman, Agus Setiyono, Aditya Wahyu Nugraha
Microalgae are gaining interest as a potential renewable energy resource for biodiesel Collagen is a type of protein that is plentiful and has a wide variety of applications. Collagen is often derived from porcine and bovine. However, both sources are considered to transmit disease, particularly porcine sources, which haram to Moslem. There are some methods for collagen extraction, one of them using the acid method. This study aimed to obtain the best extraction method and analyze collagen from alternative sources, specifically salmon skin, which is abundant. This study included two stages, i.e., pretreatment with sodium hydroxide (0.01 M, 0.03 M, 0.05 M, 0.1 M) and time (2, 4, 6, 8, 10, and 12 h) for eliminating non-collagen proteins prior from salmon skin. Moreover, the extraction was done with acetic acid (0.1 M, 0.3 M, 0.5 M) and time (24 h, 48 h and 72 h) for collagen extraction. The results indicated that the extract was collagen. It was indicated by high glycine, alanine, and proline amino acid content. Based on electrophoretic pattern, salmon skin collagen has two α chains (α1 and α2) and one α chain. The propeptides of type I procollagen have a similar composition. The most effective method for eliminating non-collagen proteins prior to extracting collagen from salmon skin was pretreatment with a 0.05 M NaOH solution for ten h. Collagen extraction using acetic acid at a concentration of 0.5 M for 48 h yielded 12.79%. © 2023, Research and Development Center for Marine and Fisheries Product Processing and Biotechnology. All rights reserved.
Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, IPB University. Dramaga Campus, West Java, Bogor, 16680, Indonesia; Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University. Dramaga Campus, West Java, Bogor, 16680, Indonesia; Division of Marine Biotechnology, Center for Coastal and Marine Resources Studies, IPB University. Dramaga Campus, West Java, Bogor, 16680, Indonesia; Departement of Agroindustrial Technology, Institut Teknologi Sumatera, Jalan Terusan Ryacudu, Way Hui, Jati Agung, South Lampung, 35365, Indonesia
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