Mustofa Ahda, Irwandi Jaswir, Deni Subara
Biopolymer-based films are created from plant or animal materials such as carbohydrates and proteins. In this study, a biopolymer-based film from gelatin and Bovine Serum Albumin (BSA) was created to form a film through cross-linking interaction of both biopolymers. The synthesized biopolymer film was characterized using Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared (FTIR) as morphological evaluation. Besides, the encapsulation efficiency of BSA-gelatin blend film was also evaluated. The coagulation process was performed using acetone carried out at 35 °C for 4 hours under stirring. The formed particles were separated from the solution using high centrifugation around 12,000 rpm for 30 min and then kept at -80°C. Based on the FTIR spectra showed that BSA and gelatin have the same functional group such as hydroxyl (OH) and Amina I, II, and III which appear at 3278 cm-1, 1633 cm-1, 1542 cm-1, and 1241 cm-1, respectively. After linkage reaction, the gelatin-BSA interaction created more strength interaction that caused no vibration functional groups seen. It also increased glass transition temperature where the resulted melting point of gelatin-based and 70% BSA/gelatin-based films are 152.2 and 260 °C, respectively. The encapsulated BSA in the blend film reached above 90% in the BSA/gelatin ratio of 70% © 2022, Carpathian Journal of Food Science and Technology.All Rights Reserved.
Department of Pharmacy, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia; Ahmad Dahlan Halal Center, Universitas Ahmad Dahlan, Yogyakarta, Indonesia; Department of Agricultural Industrial Technology, Institut Teknologi Sumatera (ITERA), Indonesia; Department of Biotechnology Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia; INHART, International Islamic University Malaysia, Kuala Lumpur, Malaysia; Department of Pharmaceutical Chemistry, International Islamic University Malaysia, Kuantan, Malaysia
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