Erma Suryanti, Iman Rusmana, Aris Tri Wahyudi, Alina Akhdiya, Deden Sukmadjaja
Expression of the genes associated with pectinase production in soft rot disease of Dickeya spp. are regulated by quorum sensing mechanism using acyl homoserine lactone (AHL). Degradation of AHL using AHL lactonase can be developed to reduce pectinase production in Dickeya spp. The aiiA gene encodes AHL lactonase from Bacillus spp. This study aims to construct binary vector of inserted aiiA gene (pCAB10) from Bacillus cereus B10 and express the aiiA gene in potato Medians cultivar genome. The aiiA gene was cloned into binary vector and transformed into Agrobacterium tumefaciens LB4404. The Agrobacterium-mediated genetic transformation was conducted with co-cultivation technique. The transgenic plant was confirmed through PCR and selected through kanamycin and soft rot disease resistance to Dickeya dadantii A3. The result showed that aiiA gene was successfully integrated to pCAB10 and introduced to A. tumefaciens LB4404. The percentage of pCAB10 transformation was 57.6%, while the regeneration efficiency was 11.9% with 50 mg/L acetosyringone addition. Four transgenic potato Medians cultivar exhibited significantly higher resistance against soft rot disease than non-transgenic plants. Mahl1 clone had the highest resistance to D. dadantii with soft rot disease incidence as 7.1% and percentage of soft rot disease suppression as 92.85%. © 2022, Society for Indonesian Biodiversity. All rights reserved.
Department of Biology, Faculty of Sciences, Institut Teknologi Sumatera, Jl. Terusan Ryacudu, Lampung Selatan, Lampung, 35365, Indonesia; Graduate Program of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Jl Agatis Kampus IPB Dramaga, West Java, Bogor, 16680, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Jl Agatis Kampus IPB Dramaga, West Java, Bogor, 16680, Indonesia; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Jl. Tentara Pelajar 3A, Cimanggu, West Java, Bogor, 16111, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock